ChimeraX Command¶
Syntax¶
circoscontacts [objects] [restrict <spec>] [outputDir <path>] [title <text>] \
[interModel true|false] [intramol true|false] \
[dnaMismatches <N>] [dnaMode auto|merge|split] \
[openHtml true|false] [keepTemp true|false]
Run usage circoscontacts inside ChimeraX for the exact installed signature.
How It Uses contacts¶
The plugin is intentionally built around ChimeraX contacts semantics.
Internally it runs contacts once per selected structure, saves those contact files, then aggregates them into the circos plot.
Default source behavior¶
If you run:
circoscontacts
then for each open structure the plugin conceptually does:
contacts <model-spec> & protein interModel false intramol false saveFile <...>
This means:
- contacts must involve
proteinon one side, - inter-model contacts are off by default,
- intramolecular contacts are off by default.
That default is deliberate: it suppresses uninformative DNA-along-DNA contact clutter.
objects¶
If you provide an object specification, that becomes the source spec, intersected per structure.
Example:
circoscontacts #1,3,4 & protein
This limits processing to the matching subset of the open models.
restrict¶
restrict is passed through in the same style as ChimeraX contacts.
Example:
circoscontacts #1,2/S,T:100-120 restrict /S,T,C interModel false intramol false
This means:
- the source side is residues
100–120of chainsS,Tin models#1,#2, - the target side is limited to chains
S,T,C, - normal
contactsrules still apply to self/intramolecular handling.
Important Aggregation Rules¶
Canonicalization¶
Displayed links are canonicalized:
- endpoint order does not matter,
- the same contact found in different models contributes to one displayed link.
Count modes¶
The HTML can display counts two ways:
Atom: every atom–atom contact row counts,Residue: each displayed residue-pair arc counts at most once per model.
For merged DNA arcs, Residue mode avoids overcounting a single model just because both nucleotides of a base pair contact the same protein residue.
DNA Handling¶
The plugin can merge DNA chains into one logical DNA arc.
dnaMode¶
auto: detect mergeable DNA layouts automaticallymerge: force merged behavior when possiblesplit: keep DNA chains separate
dnaMismatches¶
Used when detecting split/nicked dsDNA where reverse-complement matching is required.
0: exact matchN > 0: allow up toNmismatches
Output Options¶
outputDir¶
Write the generated HTML and intermediate files to a specific directory.
circoscontacts outputDir /tmp/circos_run
openHtml¶
If true, open the generated HTML automatically in the default browser.
keepTemp¶
If outputDir is not given, the plugin writes to a temporary directory. keepTemp controls whether those artifacts are retained.
Examples¶
All open structures¶
circoscontacts
Selected models only¶
circoscontacts #1,3,4 & protein
Restrict contacts to a window against defined chains¶
circoscontacts #1,2/S,T:100-120 restrict /S,T,C interModel false intramol false
Force split DNA display¶
circoscontacts dnaMode split
Keep generated files in a stable directory¶
circoscontacts outputDir ~/Desktop/circos_run title "SPO11 ensemble"
Provenance Stored In HTML¶
The generated HTML stores:
- plugin version,
- timestamp,
- reconstructed
circoscontactsinvocation, - model names,
- model filenames and paths when available.
This information is accessible from the HTML Info button.